Journal: Methods in enzymology

Article Title: Imaging the Efficacy of Anti-Inflammatory Liposomes in a Rabbit Model of Atherosclerosis by Non-Invasive Imaging

doi: 10.1016/B978-0-12-391860-4.00011-2

Figure Lengend Snippet: The induction of atherosclerosis in a New Zealand White rabbit. (A) After 2 and 6 weeks of a high cholesterol-enriched diet, a femoral angioplasty is performed by exposing the femoral artery under surgical conditions and introducing a 4-F embolectomy catheter. (B) The balloon is subsequently inflated, while pulling back the inflated balloon over the length of the aorta to denude the aorta of endothelial cells. (C) After 6 months rabbits will have advanced atherosclerotic lesions, evidenced by histology with a Masson’s trichrome stain.

Article Snippet: We use a combination of a high cholesterol diet (4.7% palm oil and 0.3% cholesterol-enriched diet, and 4.85% palm oil and 0.15% cholesterol-enriched diet; Research Diet Inc., New Jersey, USA) and a repeated balloon injury of the aorta by a femoral angioplasty under fluoroscopic guidance 2 and 6 weeks after diet initiation.

Techniques: Staining